Part:BBa_K3882000:Experience

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Applications of BBa_K3882000

How to use it? E. bsuahlscout bio-brick can transform to any competent bacterial. We use E. Coli BL21 as a recipe and safe bacterial to do the transformation. We use the LB media to culture the E. bsuahlscout. When the OD value reach to 0.2-0.4, we add 200 uM IPTG into the LB media to activate the E. bsuahlscout bio-brick inside at 30 degree centigrade for 4 hrs. After that, we make the AHL detecting kit and 1 cube is from LB-agar plate which contains 500 uM IPTG. The sample LB media which contains AHL will culture the activated E. bsuahlscout at 30 about 1-2 hrs until the red color show up.

User Reviews

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